More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| feline sarcoma oncogene | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human FES(808-822aa STIYQELQSIRKRHR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FES |
Protein | Tyrosine-protein kinase Fes/Fps |
Uniprot ID | P07332 |
Function | Tyrosine-protein kinase that acts downstream of cell surface receptors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, cell attachment and cell spreading. Plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Acts down- stream of the activated FCER1 receptor and the mast/stem cell growth factor receptor KIT. Plays a role in the regulation of mast cell degranulation. Plays a role in the regulation of cell differentiation and promotes neurite outgrowth in response to NGF signaling. Plays a role in cell scattering and cell migration in response to HGF-induced activation of EZR. Phosphorylates BCR and down-regulates BCR kinase activity. Phosphorylates HCLS1/HS1, PECAM1, STAT3 and TRIM28. |
Tissue Specificity | Widely expressed. Detected in adult colon epithelium. |
Sub-cellular localization | Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle. Golgi apparatus. Cell junction, focal adhesion. Note: Distributed throughout the cytosol when the kinase is not activated. Association with microtubules requires activation of the kinase activity. Shuttles between focal adhesions and cell-cell contacts in epithelial cells. Recruited to the lateral cell membrane in polarized epithelial cells by interaction with phosphorylated EZR. Detected at tubular membrane structures in the cytoplasm and at the cell periphery. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily. |
Aliases | c-fes/fps protein antibody|cFES antibody|feline sarcoma(Snyder-Theilen) viral(v-fes)/Fujinami antibody|Feline sarcoma oncogene antibody|fes antibody|FES_HUMAN antibody|FPS antibody|Oncogene FES antibody|Oncogene FES feline sarcoma virus antibody|Proto oncogene tyrosine protein kinase Fes/Fps antibody|Proto-oncogene c-Fes antibody|Proto-oncogene c-Fps antibody|proto-oncogene tyrosine-protein kinase Fes/Fps antibody|Tyrosine-protein kinase Fes/Fps antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FES antibody, ASA-B0696, Western blotting
All lanes: Anti FES (ASA-B0696) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 93KD
Observed bind size: 93KD
All lanes: Anti FES (ASA-B0696) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 93KD
Observed bind size: 93KD
