More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Fas cell surface death receptor | Polyclonal | IgG | Rabbit | Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived rat Fas recombinant protein (Position: Q22-E324). Rat Fas shares 52% and 61% amino acid (aa) sequences identity with human and mouse Fas, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FAS |
Protein | Tumor necrosis factor receptor superfamily member 6 |
Uniprot ID | Q63199 |
Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 death domain. |
Aliases | ALPS 1A antibody|ALPS1A antibody|APO 1 antibody|Apo 1 antigen antibody|APO 1 cell surface antigen antibody|Apo-1 antigen antibody|APO1 antibody|Apo1 antigen antibody|APO1 cell surface antigen antibody|Apoptosis antigen 1 antibody|Apoptosis mediating surface antigen FAS antibody|Apoptosis-mediating surface antigen FAS antibody|APT 1 antibody|APT1 antibody|CD 95 antibody|CD 95 antigen antibody|CD95 antibody|CD95 antigen antibody|Delta Fas antibody|Delta Fas/APO 1/CD95 antibody|Delta Fas/APO1/CD95 antibody|Fas (TNF receptor superfamily, member 6) antibody|FAS 1 antibody|FAS 827dupA antibody|Fas AMA antibody|FAS antibody|FAS Antigen antibody|Fas cell surface death receptor antibody|FAS1 antibody|FASLG receptor antibody|FASTM antibody|sFAS antibody|Surface antigen APO1 antibody|TNF receptor superfamily, member 6 antibody|TNFRSF 6 antibody|TNFRSF6 antibody|TNR6_HUMAN antibody|Tumor necrosis factor receptor superfamily member 6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Fas antibody, ASA-B0683, Western blotting
All lanes: Anti Fas (ASA-B0683) at 0.5ug/ml
WB: Recombinant Rat Fas Protein 0.5ng
Predicted bind size: 35KD
Observed bind size: 35KD
All lanes: Anti Fas (ASA-B0683) at 0.5ug/ml
WB: Recombinant Rat Fas Protein 0.5ng
Predicted bind size: 35KD
Observed bind size: 35KD
Anti- Fas antibody, ASA-B0683, Western blotting
All lanes: Anti Fas (ASA-B0683) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti Fas (ASA-B0683) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
