More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Fas(TNFRSF6) associated factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human FAF1(1-22aa MASNMDREMILADFQACTGIEN), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FAF1 |
Protein | FAS-associated factor 1(hFAF1) |
Uniprot ID | Q9UNN5 |
Function | Potentiates but cannot initiate FAS-induced apoptosis. |
Tissue Specificity | Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes. |
Sub-cellular localization | Nucleus . |
Sequence Similarities | Contains 1 UBA domain. |
Aliases | CGI 03 antibody|FAF1 antibody|FAF1 protein antibody|FAF1_HUMAN antibody|Fas(TNFRSF6) associated factor 1 antibody|FAS associated factor 1 antibody|Fas associated protein factor 1 antibody|FAS-associated factor 1 antibody|FLJ37524 antibody|hFAF1 antibody|HFAF1s antibody|Protein FAF1 antibody|TNFRSF6 associated factor 1 antibody|UBX domain protein 3A antibody|UBX domain-containing protein 12 antibody|UBX domain-containing protein 3A antibody|UBXD12 antibody|UBXN3A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FAF1 antibody, ASA-B0680, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: SW620 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: SW620 Cell Lysate
