More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| coagulation factor VIII, procoagulant component | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Factor VIII(1264-1278aa APVLQDFRSLNDSTN). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | F8 |
Protein | Coagulation factor VIII |
Uniprot ID | P00451 |
Function | Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space. |
Sequence Similarities | Belongs to the multicopper oxidase family. |
Aliases | Ahf antibody|Antihemophilic factor antibody|Coagulation factor VIII antibody|Coagulation factor VIII associated protein b antibody|Coagulation factor VIII isoform b antibody|Coagulation factor VIII procoagulent component antibody|coagulation factor VIII, procoagulant component antibody|Coagulation factor VIIIc antibody|Dna segment on chromosome x unique 1253 expressed sequence antibody|Dxs1253e antibody|F8 antibody|F8 protein antibody|F8b antibody|F8c antibody|FA8_HUMAN antibody|Factor VIII F8b antibody|Factor VIIIa light chain antibody|FactorVIII antibody|FVIII antibody|Hema antibody|Hema coagulation factor VIIIc procoagulent component antibody|Hemophilia a antibody|Hemophilia classic antibody|Hemophilia, classic antibody|OTTHUMP00000061446 antibody|OTTHUMP00000196174 antibody|Procoagulant component antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Factor VIII antibody, ASA-B0678, Western blotting
Lane 1: A431 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: RAJI Cell Lysate
Lane 6: CEM Cell Lysate
Lane 7: HL-60 Cell Lysate
Lane 8: MCF-7 Cell Lysate
Lane 1: A431 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: RAJI Cell Lysate
Lane 6: CEM Cell Lysate
Lane 7: HL-60 Cell Lysate
Lane 8: MCF-7 Cell Lysate
