More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| coagulation factor VII(serum prothrombin conversion accelerator) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Factor VII(441-453aa SQYIEWLQKLMRS). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | F7 |
Protein | Coagulation factor VII |
Uniprot ID | P08709 |
Function | Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. |
Tissue Specificity | Plasma. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the peptidase S1 family. |
Aliases | coagulation factor VII(serum prothrombin conversion accelerator) antibody|Coagulation factor VII antibody|Eptacog alfa antibody|F7 antibody|FA7_HUMAN antibody|Factor VII antibody|Factor VII heavy chain antibody|Factor VII light chain antibody|FVII coagulation protein antibody|OTTHUMP00000018733 antibody|OTTHUMP00000018734 antibody|Proconvertin antibody|Serum prothrombin conversion accelerator antibody|SPCA antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Factor VII antibody, ASA-B0677, Western blotting
All lanes: Anti Factor VII (ASA-B0677) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 52KD
All lanes: Anti Factor VII (ASA-B0677) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 52KD
