More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mitogen-activated protein kinase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ERK2(324-335aa IAEAPFKFDMEL), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAPK1 |
Protein | Mitogen-activated protein kinase 1 |
Uniprot ID | P28482 |
Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm, cytoskeleton, spindle . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Note: Associated with the spindle during prometaphase and metaphase (By similarity). PEA15- binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization. |
Sequence Similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. |
Aliases | ERK 2 antibody|ERK antibody|ERK-2 antibody|ERT 1 antibody|ERT1 antibody|Extracellular Signal Regulated Kinase 2 antibody|Extracellular signal-regulated kinase 2 antibody|MAP kinase 1 antibody|MAP kinase 2 antibody|MAP kinase 2 antibody|MAP kinase isoform p42 antibody|MAPK 1 antibody|MAPK 2 antibody|MAPK 2 antibody|MAPK1 antibody|MAPK2 antibody|Mitogen Activated Protein Kinase 1 antibody|Mitogen Activated Protein Kinase 2 antibody|Mitogen-activated protein kinase 1 antibody|Mitogen-activated protein kinase 2 antibody|MK01_HUMAN antibody|P38 antibody|P40 antibody|P41 antibody|P41MAPK antibody|p42 MAPK antibody|p42-MAPK antibody|P42MAPK antibody|P42MAPK antibody|PRKM 1 antibody|PRKM 2 antibody|PRKM1 antibody|PRKM2 antibody|Protein Tyrosine Kinase ERK2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ERK2 antibody, ASA-B0666, All Western blotting
All lanes: Anti-MAPK1(ASA-B0666) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 40ug
Lane 2: Rat Liver Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: Rat Thymus Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: MM231 Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysate at
All lanes: Anti-MAPK1(ASA-B0666) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 40ug
Lane 2: Rat Liver Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: Rat Thymus Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: MM231 Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysate at
