More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| erythropoietin receptor | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human EPO Receptor(362-379aa EHAQDTYLVLDKWLLPRN), identical to the related mouse sequence, and different from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EPOR |
Protein | Erythropoietin receptor |
Uniprot ID | P19235 |
Function | Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. |
Tissue Specificity | Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early- stage erythroid progenitor cells. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the type I cytokine receptor family. Type 1 subfamily. |
Aliases | EPO R antibody|EPO-R antibody|epor antibody|EPOR_HUMAN antibody|Erythropoietin receptor antibody|Erythropoietin receptor precursor antibody|MGC138358 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EPO Receptor antibody, ASA-B0661, Western blotting
All lanes: Anti EPO Receptor (ASA-B0661) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 55KD
Observed bind size: 55KD
All lanes: Anti EPO Receptor (ASA-B0661) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 55KD
Observed bind size: 55KD
