More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| erythrocyte membrane protein band 4.1-like 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human EPB41L1 recombinant protein (Position: Q691-S881). Human EPB41L1 shares 91% amino acid (aa) sequence identity with both mouse and rat EPB41L1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EPB41L1 |
Protein | Band 4.1-like protein 1 |
Uniprot ID | Q9H4G0 |
Function | May function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases. |
Tissue Specificity | Highest expression in brain, lower in heart, kidney, pancreas, placenta, lung and skeletal muscle. |
Sub-cellular localization | Cytoplasm, cytoskeleton. |
Sequence Similarities | Contains 1 FERM domain. |
Aliases | 4.1N antibody|Band 4.1 like protein 1 antibody|Band 4.1-like protein 1 antibody|DKFZp686H17242 antibody|E41L1_HUMAN antibody|EPB41L 1 antibody|Epb41l1 antibody|Erythrocyte membrane protein band 4.1 like 1 antibody|KIAA0338 antibody|MGC11072 antibody|MRD11 antibody|Neuron type nonerythroid protein 4.1 antibody|Neuronal protein 4.1 antibody|Nonerythroid protein 4.1 antibody|Nonerythroid protein 4.1, neuron type antibody|OTTHUMP00000030825 antibody|OTTHUMP00000030826 antibody|OTTHUMP00000030827 antibody|OTTHUMP00000030829 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- EPB41L1 antibody, ASA-B0654, Western blotting
All lanes: Anti EPB41L1 (ASA-B0654) at 0.5ug/ml
WB: Recombinant Human EPB41L1 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
All lanes: Anti EPB41L1 (ASA-B0654) at 0.5ug/ml
WB: Recombinant Human EPB41L1 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Anti- EPB41L1 antibody, ASA-B0654, Western blotting
All lanes: Anti EPB41L1 (ASA-B0654) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 98KD
Observed bind size: 98KD
All lanes: Anti EPB41L1 (ASA-B0654) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 98KD
Observed bind size: 98KD
