More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| endomucin | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Endomucin (225-258aa ENGNDQPQSDKESVKLLTVKTISHESGEHSAQGK), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EMCN |
Protein | Endomucin |
Uniprot ID | Q9ULC0 |
Function | Endothelial sialomucin, also called endomucin or mucin- like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. |
Tissue Specificity | Expressed in heart, kidney and lung. |
Sub-cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Note: Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks. |
Sequence Similarities | |
Aliases | EMCN antibody|EMCN2 antibody|Endomucin 2 antibody|Endomucin antibody|Endomucin-2 antibody|Endothelial sialomucin antibody|Gastric cancer antigen Ga34 antibody|MUC-14 antibody|MUC14 antibody|MUCEN_HUMAN antibody|Mucin 14 antibody|Mucin-14 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Endomucin antibody, ASA-B0645, Western blotting
All lanes: Anti Endomucin (ASA-B0645) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
All lanes: Anti Endomucin (ASA-B0645) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
