More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| eukaryotic translation initiation factor 3, subunit B | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B(755-775aa EDFRKYRKMAQELYMEQKNER), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EIF3B |
Protein | Eukaryotic translation initiation factor 3 subunit B |
Uniprot ID | P55884 |
Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the eIF-3 subunit B family. |
Aliases | eIF-3-eta antibody|eIF3 eta antibody|eIF3 p110 antibody|eIF3 p116 antibody|EIF3-ETA antibody|EIF3-P110 antibody|EIF3-P116 antibody|eIF3b antibody|EIF3B_HUMAN antibody|EIF3S9 antibody|Eukaryotic translation initiation factor 3, subunit B antibody|Eukaryotic translation initiation factor 3 subunit 9 antibody|eukaryotic translation initiation factor 3 subunit 9 eta antibody|Eukaryotic translation initiation factor 3 subunit B antibody|hPrt1 antibody|PRT1 antibody|Prt1 homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Eif3b antibody, ASA-B0642, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: A431 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: A431 Cell Lysate
