More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| eukaryotic translation initiation factor 2-alpha kinase 1 | Polyclonal | IgG | Rabbit | Human | ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human EIF2AK1(466-483aa CTDILQKNTDWTNRNGKR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EIF2AK1 |
Protein | Eukaryotic translation initiation factor 2-alpha kinase 1 |
Uniprot ID | Q9BQI3 |
Function | Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity). |
Tissue Specificity | Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level). |
Sub-cellular localization | Cytoplasm . |
Sequence Similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. |
Aliases | E2AK1_HUMAN antibody|EC 2.7.11.1 antibody|Eif2ak1 antibody|Eukaryotic translation initiation factor 2 alpha kinase 1 antibody|Eukaryotic translation initiation factor 2-alpha kinase 1 antibody|HCR antibody|Heme controlled repressor antibody|Heme regulated eukaryotic initiation factor eIF 2 alpha kinase antibody|Heme regulated inhibitor antibody|Heme regulated initiation factor 2 alpha kinase antibody|Heme regulated repressor antibody|Heme sensitive initiation factor 2a kinase antibody|Heme-controlled repressor antibody|Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase antibody|Heme-regulated inhibitor antibody|Hemin sensitive initiation factor 2 alpha kinase antibody|Hemin-sensitive initiation factor 2-alpha kinase antibody|HRI antibody|KIAA1369 antibody|PRO1362 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EIF2AK1 antibody, ASA-B0639, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK1, 35.5KD(162aa tag+ R398-K551)
Lane 1: Recombinant Human EIF2AK1 Protein 10ng
Lane 2: Recombinant Human EIF2AK1 Protein 5ng
Lane 3: Recombinant Human EIF2AK1 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK1, 35.5KD(162aa tag+ R398-K551)
Lane 1: Recombinant Human EIF2AK1 Protein 10ng
Lane 2: Recombinant Human EIF2AK1 Protein 5ng
Lane 3: Recombinant Human EIF2AK1 Protein 2.5ng
Anti-EIF2AK1 antibody, ASA-B0639, ICC
ICC: HELA Cell
ICC: HELA Cell
