More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ectodysplasin A | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human EDA recombinant protein (Position: A30-S391). Human EDA shares 95% amino acid (aa) sequence identity with mouse EDA. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EDA |
Protein | Ectodysplasin-A |
Uniprot ID | Q92838 |
Function | Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. |
Tissue Specificity | Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord. |
Sub-cellular localization | Cell membrane; Single-pass type II membrane protein. |
Sequence Similarities | Belongs to the tumor necrosis factor family. |
Aliases | ECTD1 antibody|Ectodermal dysplasia 1, anhidrotic antibody|Ectodermal dysplasia protein antibody|Ectodermal dysplasia, anhidrotic (hypohydrotic) antibody|Ectodysplasin A antibody|Ectodysplasin A, membrane form antibody|Ectodysplasin A, secreted form antibody|ECTODYSPLASIN A1 ISOFORM antibody|ECTODYSPLASIN A2 ISOFORM antibody|ECTODYSPLASIN antibody|Ectodysplasin-A antibody|ED1 A1 antibody|ED1 A2 antibody|ED1 antibody|ED1 GENE antibody|Eda A1 antibody|Eda A2 antibody|eda antibody|EDA protein antibody|EDA protein homolog antibody|EDA_HUMAN antibody|EDA1 antibody|EDA1 GENE antibody|EDA2 antibody|HED antibody|HED1 antibody|ODT1 antibody|Oligodontia 1 antibody|secreted form antibody|STHAGX1 antibody|Ta antibody|Tabby antibody|Tabby protein antibody|X linked anhidroitic ectodermal dysplasia protein antibody|XHED antibody|XLHED antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- EDA antibody, ASA-B0632, Western blotting
All lanes: Anti EDA (ASA-B0632) at 0.5ug/ml
WB: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti EDA (ASA-B0632) at 0.5ug/ml
WB: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
Anti- EDA antibody, ASA-B0632, Western blotting
All lanes: Anti EDA (ASA-B0632) at 0.5ug/ml
WB : Recombinant Human EDA Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD
All lanes: Anti EDA (ASA-B0632) at 0.5ug/ml
WB : Recombinant Human EDA Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD
