More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 1(high affinity aspartate/glutamate transporter), member 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT4(516-533aa AAVIEHLSQRELELQEAE), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC1A6 |
Protein | Excitatory amino acid transporter 4 |
Uniprot ID | P48664 |
Function | Transports L-glutamate and also L- and D-aspartate. Seems to act as a symport by cotransporting sodium. |
Tissue Specificity | Brain. Expressed densely and selectively in cell bodies of Purkinje cells. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | Excitatory amino acid transporter 4 antibody|High affinity neuronal glutamate transporter antibody|High-affinity neuronal glutamate transporter antibody|MGC33092 antibody|MGC43671 antibody|Slc1a6 antibody|Sodium dependent glutamate/aspartate transporter antibody|Sodium-dependent glutamate/aspartate transporter antibody|Solute carrier family 1(high affinity aspartate/glutamate transporter) member 6 antibody|Solute carrier family 1 member 6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EAAT4 antibody, ASA-B0629, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: NRK Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: NRK Cell Lysate
