More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 1(neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse EAAT3(10-27aa DWRRFLRNHWLLLSTVAA), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC1A1 |
Protein | Excitatory amino acid transporter 3 |
Uniprot ID | P51906 |
Function | Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium (By similarity). Negatively regulated by ARL6IP5 (PubMed:12119102). |
Tissue Specificity | Expressed in brain, lung, kidney and skeletal muscle. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | |
Aliases | EAA3_HUMAN antibody|EAAC 1 antibody|EAAC1 antibody|EAAT 3 antibody|Excitatory amino acid carrier 1 antibody|Excitatory amino acid carrier1 antibody|Excitatory amino acid transporter 3 antibody|Excitatory amino acid transporter3 antibody|Excitatory amino-acid carrier 1 antibody|GLUTAMATE TRANSPORTER, HIGH-AFFINITY antibody|MEAAC 1 antibody|MEAAC1 antibody|Neuronal and epithelial glutamate transporter antibody|REAAC 1 antibody|REAAC1 antibody|Slc1 a1 antibody|Slc1a 1 antibody|SLC1A1 antibody|Sodium dependent glutamate/aspartate transporter 3 antibody|Sodium-dependent glutamate/aspartate transporter 3 antibody|Solute carrier family 1(neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody|SOLUTE CARRIER FAMILY 1(NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1 antibody|Solute carrier family 1(neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody|Solute carrier family 1 member 1 antibody|Solute carrier family 1, member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EAAT3 antibody, ASA-B0628, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: HEPA Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: HEPA Cell Lysate
