More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 1(neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
| A synthetic peptide corresponding to a sequence at the N-terminus of human EAAT3(10-27aa EWKRFLKNNWVLLSTVAA). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC1A1 |
Protein | Excitatory amino acid transporter 3 |
Uniprot ID | P43005 |
Function | Transports L-glutamate, L- and D-aspartate and L-cystein (PubMed:21123949). Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5 (By similarity). |
Tissue Specificity | Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | |
Aliases | EAA3_HUMAN antibody|EAAC 1 antibody|EAAC1 antibody|EAAT 3 antibody|Excitatory amino acid carrier 1 antibody|Excitatory amino acid carrier1 antibody|Excitatory amino acid transporter 3 antibody|Excitatory amino acid transporter3 antibody|Excitatory amino-acid carrier 1 antibody|GLUTAMATE TRANSPORTER, HIGH-AFFINITY antibody|MEAAC 1 antibody|MEAAC1 antibody|Neuronal and epithelial glutamate transporter antibody|REAAC 1 antibody|REAAC1 antibody|Slc1 a1 antibody|Slc1a 1 antibody|SLC1A1 antibody|Sodium dependent glutamate/aspartate transporter 3 antibody|Sodium-dependent glutamate/aspartate transporter 3 antibody|Solute carrier family 1(neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody|SOLUTE CARRIER FAMILY 1(NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1 antibody|Solute carrier family 1(neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody|Solute carrier family 1 member 1 antibody|Solute carrier family 1, member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EAAT3 antibody, ASA-B0627, Western blotting
WB: Human Placenta Tissue Lysate
