More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| protein inhibitor of activated STAT, 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human E3 SUMO-protein ligase PIAS4(295-310aa HPELCKALVKEKLRLD), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PIAS4 |
Protein | E3 SUMO-protein ligase PIAS4 |
Uniprot ID | Q8N2W9 |
Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. |
Tissue Specificity | Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. |
Sub-cellular localization | Nucleus, PML body. |
Sequence Similarities | Belongs to the PIAS family. |
Aliases | E3 SUMO protein ligase PIAS4 antibody|E3 SUMO-protein ligase PIAS4 antibody|FLJ12419 antibody|MGC35296 antibody|PAIASgamma antibody|PIAS 4 antibody|PIAS gamma antibody|PIAS-gamma antibody|PIAS4 antibody|PIAS4_HUMAN antibody|PIASG antibody|PIASgamma antibody|PIASy antibody|Protein inhibitor of activated STAT 4 antibody|Protein inhibitor of activated STAT protein 4 antibody|Protein inhibitor of activated STAT protein gamma antibody|Protein inhibitor of activated STAT protein PIASy antibody|Zinc finger MIZ type containing 6 antibody|ZMIZ 6 antibody|ZMIZ6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PIAS4 antibody, ASA-B0624, Western blotting
All lanes: Anti PIAS4 (ASA-B0624) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: A431 Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
All lanes: Anti PIAS4 (ASA-B0624) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: A431 Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
