More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor necrosis factor receptor superfamily, member 25 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human DR3(73-94aa CPQDTFLAWENHHNSECARCQA). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNFRSF25 |
Protein | Tumor necrosis factor receptor superfamily member 25 |
Uniprot ID | Q93038 |
Function | Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. |
Tissue Specificity | Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. |
Sub-cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 death domain. |
Aliases | Apo 3 antibody|Apo-3 antibody|Apo3 antibody|Apoptosis inducing receptor AIR antibody|Apoptosis inducing receptor antibody|Apoptosis mediating receptor antibody|Apoptosis mediating receptor DR 3 antibody|Apoptosis mediating receptor DR3 antibody|Apoptosis mediating receptor TRAMP antibody|Apoptosis-inducing receptor AIR antibody|Apoptosis-mediating receptor DR3 antibody|Apoptosis-mediating receptor TRAMP antibody|DDR 3 antibody|DDR3 antibody|Death domain receptor 3 antibody|Death Receptor 3 antibody|Death receptor beta antibody|DR 3 antibody|LARD antibody|Lymphocyte associated receptor of death antibody|Lymphocyte-associated receptor of death antibody|Protein WSL antibody|Protein WSL-1 antibody|TNFRSF 12 antibody|TNFRSF 25 antibody|TNFRSF12 antibody|TNFRSF25 antibody|TNR25_HUMAN antibody|TR 3 antibody|TR3 antibody|TRAMP antibody|Translocating chain association membrane protein antibody|Tumor necrosis factor receptor superfamily member 12 antibody|Tumor necrosis factor receptor superfamily member 25 antibody|WSL 1 antibody|WSL antibody|WSL LR antibody|WSL protein antibody|WSL1 antibody|WSL1 protein antibody|WSLLR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- DR3 antibody, ASA-B0608, Western blotting
All lanes: Anti DR3 (ASA-B0608) at 0.5ug/ml
WB: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
All lanes: Anti DR3 (ASA-B0608) at 0.5ug/ml
WB: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
