More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
delta-like 1(Drosophila) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of human DLL1(168-184aa RTDLKYSYRFVCDEHYY), different from the related rat and mouse sequences by one amino acid. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DLL1 |
Protein | Delta-like protein 1 |
Uniprot ID | O00548 |
Function | Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. |
Tissue Specificity | Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 DSL domain. |
Aliases | Delta(Drosophila) like 1 antibody|Delta antibody|Delta like 1(Drosophila) antibody|Delta like 1 antibody|Delta like 1 protein antibody|Delta like protein 1 antibody|Delta like protein 1 precursor antibody|Delta, Drosophila, homolog of 1 antibody|Delta-like protein 1 [Precursor] antibody|Delta-like protein 1 antibody|Delta1 antibody|DL1 antibody|DLL 1 antibody|Dll1 antibody|DLL1_HUMAN antibody|Drosophila Delta homolog 1 antibody|H-Delta-1 antibody|OTTHUMP00000017690 antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DLL1 antibody, ASA-B0598, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human DLL1, 39.9KD(162aa tag+M1-G200)
Lane 1: Recombinant Human DLL1 Protein 10ng
Lane 2: Recombinant Human DLL1 Protein 5ng
Lane 3: Recombinant Human DLL1 Protein 2.5ng
Lane 4: Recombinant Human DLL1 Protein 1.25ng