More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| dickkopf WNT signaling pathway inhibitor 2 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human DKK2 recombinant protein (Position: K34-I259). Human DKK2 shares 96% amino acid (aa) sequence identity with mouse DKK2. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DKK2 |
Protein | Dickkopf-related protein 2 |
Uniprot ID | Q9UBU2 |
Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). |
Tissue Specificity | Expressed in heart, brain, skeletal muscle and lung. |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | Dickkopf 2 antibody|Dickkopf gene 2 antibody|Dickkopf homolog 2 antibody| Dickkopf related protein 2 antibody|Dickkopf WNT signaling pathway inhibitor 2 antibody|Dickkopf-2 antibody| Dickkopf-related protein 2 antibody|Dickkopf2 antibody|DKK 2 antibody|Dkk-2 antibody|Dkk2 antibody|DKK2_HUMAN antibody| hDkk 2 antibody|hDkk-2 antibody|hDkk2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- DKK2 antibody, ASA-B0595, Western blotting
All lanes: Anti DKK2 (ASA-B0595) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
All lanes: Anti DKK2 (ASA-B0595) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
