More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| dickkopf 1 homolog(Xenopus laevis) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human DKK1(68-83aa YPGGNKYQTIDNYQPY), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DKK1 |
Protein | Dickkopf-related protein 1(Dickkopf-1/Dkk-1/hDkk-1) |
Uniprot ID | O94907 |
Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. |
Tissue Specificity | Placenta. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the dickkopf family. |
Aliases | Dickkopf 1 antibody|Dickkopf 1 homolog antibody|Dickkopf 1 like antibody|Dickkopf homolog 1 antibody|Dickkopf like protein 1 antibody|Dickkopf related protein 1 antibody|Dickkopf-1 antibody|Dickkopf-related protein 1 antibody|DKK 1 antibody|Dkk-1 antibody|Dkk1 antibody|DKK1_HUMAN antibody|hDkk 1 antibody|hDkk-1 antibody|hDkk1 antibody|SK antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DKK1 antibody, ASA-B0594, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: A549 Cell Lysate
Lane 1: U87 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: A549 Cell Lysate
