More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytoglobin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Cytoglobin(50-68aa FVNFPSAKQYFSQFKHMED), identical to the related rat sequence, different from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYGB |
Protein | Cytoglobin |
Uniprot ID | Q8WWM9 |
Function | May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer. |
Tissue Specificity | Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. |
Sub-cellular localization | Cytoplasm . |
Sequence Similarities | |
Aliases | Cygb antibody|CYGB_HUMAN antibody|Cytoglobin antibody|Hgb antibody|Histoglobin antibody|STAP antibody|Stellate cell activation associated protein antibody|Stellate cell activation-associated protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cytoglobin antibody, ASA-B0566, Western blotting
All lanes: Anti Cytoglobin (ASA-B0566) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Small Intestine Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: SGC Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: PANC Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Ly
All lanes: Anti Cytoglobin (ASA-B0566) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Small Intestine Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: SGC Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: PANC Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Ly
