More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytochrome P450, family 7, subfamily A, polypeptide 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CYP7A1 recombinant protein (Position: M301-L504). Human CYP7A1 shares 85.2 % and 83.3% amino acid (aa) sequence identity with mouse and rat CYP7A1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYP7A1 |
Protein | Cholesterol 7-alpha-monooxygenase |
Uniprot ID | P22680 |
Function | Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. |
Tissue Specificity | Detected in liver. |
Sub-cellular localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
Sequence Similarities | Belongs to the cytochrome P450 family. |
Aliases | Cholesterol 7 alpha hydroxylase antibody|Cholesterol 7 alpha monooxygenase antibody| cholesterol 7 alpha-monooxygenase antibody|Cholesterol 7-alpha-hydroxylase antibody| Cholesterol 7-alpha-monooxygenase antibody|CP7A antibody|CP7A1_HUMAN antibody|CYP 7 antibody|CYP7 antibody|CYP7A1 antibody|CYPVII antibody|Cytochrome P450 7A1 antibody| cytochrome P450, family 7, subfamily A, polypeptide 1 antibody|Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody|cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), antibody|Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody|MGC126826 antibody|MGC138389 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CYP7A1 antibody, ASA-B0552, Western blotting
All lanes: Anti CYP7A1 (ASA-B0552) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 58KD
Observed bind size: 58KD
All lanes: Anti CYP7A1 (ASA-B0552) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 58KD
Observed bind size: 58KD
