More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytochrome P450, family 11, subfamily B, polypeptide 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11B1(436-450aa RNFYHVPFGFGMRQC). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYP11B1 |
Protein | Cytochrome P450 11B1, mitochondrial |
Uniprot ID | P15538 |
Function | Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion membrane. |
Sequence Similarities | |
Aliases | CPN1 antibody|CYP11B antibody|CYPXIB1 antibody|Cytochrome P 450c11 antibody|Cytochrome P450 11B1, mitochondrial antibody|Cytochrome p450 XIB1 antibody|Cytochrome P450, family 11, subfamily B, polypeptide 1 antibody|Cytochrome P450, subfamily XIB(steroid 11 beta hydroxylase), polypeptide 1 antibody|Cytochrome P450C11 antibody|FHI antibody|P 450C11 antibody|P450C11 antibody|S11BH antibody|Steroid 11 beta hydroxylase antibody|Steroid 11 beta monooxygenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CYP11B1 antibody, ASA-B0543, Western blotting
All lanes: Anti CYP11B1 (ASA-B0543) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
All lanes: Anti CYP11B1 (ASA-B0543) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
