More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine(C-X-C motif) receptor 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human CXCR2(196-212aa CYEDMGNNTANWRMLLR), different from the related mouse sequence by six amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CXCR2 |
Protein | C-X-C chemokine receptor type 2(CXC-R2/CXCR-2) |
Uniprot ID | P25025 |
Function | Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the G-protein coupled receptor 1 family. |
Aliases | C-X-C chemokine receptor type 2 antibody|CD 182 antibody|CD182 antibody|CD182 antigen antibody|CDw128b antibody|Chemokine(CXC) receptor 2 antibody|CMKAR2 antibody|CXC-R2 antibody|CXCR 2 antibody|CXCR-2 antibody|CXCR2 antibody|CXCR2_HUMAN antibody|GRO/MGSA receptor antibody|High affinity interleukin-8 receptor B antibody|IL 8 receptor type 2 antibody|IL 8R B antibody|IL-8 receptor type 2 antibody|IL-8R B antibody|IL8 RB antibody|IL8 receptor type 2 antibody|IL8R B antibody|IL8R2 antibody|IL8RA antibody|Interleukin 8 Receptor B antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CXCR2 antibody, ASA-B0521, Western blotting
Lane 1: Human Rectal Cancer Tissue Lysate
Lane 2: Human Rectal Cancer Tissue Lysate
Lane 1: Human Rectal Cancer Tissue Lysate
Lane 2: Human Rectal Cancer Tissue Lysate
Anti- CXCR2 antibody, ASA-B0521, Western blotting
All lanes: Anti CXCR2(ASA-B0521) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Lane 6: Mouse BrainTissue Lysate at 50ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti CXCR2(ASA-B0521) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Lane 6: Mouse BrainTissue Lysate at 50ug
Predicted bind size: 41KD
Observed bind size: 41KD
