More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine(C-X3-C motif) ligand 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CX3CL1(50-64aa HYQQNQASCGKRAII). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CX3CL1 |
Protein | Fractalkine |
Uniprot ID | P78423 |
Function | The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. |
Tissue Specificity | Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the intercrine delta family. |
Aliases | A 152E5.2 antibody|AB030188 antibody|ABCD 3 antibody|ABCD3 antibody|AI848747 antibody|C-X3-C motif chemokine 1 antibody|C3Xkine antibody|Chemokine(C-X3-C motif) ligand 1 antibody|Chemokine C X3 C motif ligand 1 antibody|Chemokine CX3C Motif Ligand 1 antibody|CX3C membrane anchored chemokine antibody|CX3C membrane-anchored chemokine antibody|CX3CL1 antibody|CXC 3 antibody|CXC3 antibody|CXC3C antibody|D8Bwg0439e antibody|FKN antibody|Fractalkine antibody|Neurotactin antibody|NTN antibody|NTT antibody|Processed fractalkine antibody|SCYD 1 antibody|SCYD1 antibody|Small inducible cytokine D1 antibody|Small inducible cytokine subfamily D(Cys X3 Cys) member 1 antibody|small inducible cytokine subfamily D(Cys-X3-Cys), member 1(fractalkine, neurotactin) antibody|Small inducible cytokine subfamily D member 1 antibody|Small-inducible cytokine D1 antibody|X3CL1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CX3CL1 antibody, ASA-B0517, Western blotting
Lane 1: Recombinant Human Fractalkine Protein 10ng
Lane 2: Recombinant Human Fractalkine Protein 5ng
Lane 3: Recombinant Human Fractalkine Protein 2.5ng
Lane 1: Recombinant Human Fractalkine Protein 10ng
Lane 2: Recombinant Human Fractalkine Protein 5ng
Lane 3: Recombinant Human Fractalkine Protein 2.5ng
