More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cullin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Cullin 2(264-280aa HECQQRMVADHLQFLHA), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CUL2 |
Protein | Cullin-2 |
Uniprot ID | Q13617 |
Function | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | |
Aliases | Cul2 antibody|CUL 2 antibody|CUL-2 antibody|CUL2 antibody|CUL2_HUMAN antibody|cullin 2 antibody|cullin homolog 2 antibody|Cullin-2 antibody|MGC131970 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cullin 2 antibody, ASA-B0513, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human Cul2, 46.5KD(162aa tag+L150-Y394)
Lane 1: Recombinant Human Cul2 Protein 10ng
Lane 2: Recombinant Human Cul2 Protein 5ng
Lane 3: Recombinant Human Cul2 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant human Cul2, 46.5KD(162aa tag+L150-Y394)
Lane 1: Recombinant Human Cul2 Protein 10ng
Lane 2: Recombinant Human Cul2 Protein 5ng
Lane 3: Recombinant Human Cul2 Protein 2.5ng
Anti-Cullin 2 antibody, ASA-B0513, Western blotting
WB: Rat Brain Tissue Lysate
WB: Rat Brain Tissue Lysate
Anti-Cullin 2 antibody, ASA-B0513, Western blotting
Lane 1: A431 Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 1: A431 Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: COLO320 Cell Lysate