More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cAMP responsive element binding protein 3-like 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CREB3L1 recombinant protein (Position: M1-M204). Human CREB3L1 shares 93.6% and 93.1% amino acid (aa) sequence identity with mouse and rat CREB3L1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CREB3L1 |
Protein | Cyclic AMP-responsive element-binding protein 3-like protein 1 |
Uniprot ID | Q96BA8 |
Function | Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. Directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. Does not regulate COL1A1 in other tissues, such as skin (By similarity). Required to protect astrocytes from ER stress-induced cell death. In astrocytes, binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation (By similarity). May play a role in limiting virus spread by inhibiting proliferation of virus-infected cells. Upon infection with diverse DNA and RNA viruses, inhibits cell-cycle progression by binding to promoters and activating transcription of genes encoding cell-cycle inhibitors, such as p21/CDKN1A (PubMed:21767813). Binds the DNA consensus sequence 5'-GTGXGCXGC- 3' (PubMed:21767813). |
Tissue Specificity | Expressed in several tissues, with highest levels in pancreas and prostate. Expressed at relatively lower levels in brain. |
Sub-cellular localization | Endoplasmic reticulum membrane. |
Sequence Similarities | Belongs to the bZIP family. ATF subfamily. |
Aliases | BBF-2 homolog antibody|CAMP responsive element binding protein 3 like 1 antibody|cAMP-responsive element-binding protein 3-like protein 1 antibody| CR3L1_HUMAN antibody|Creb3l1 antibody|Cyclic AMP responsive element binding protein 3 like protein 1 antibody|OASIS antibody|Old astrocyte specifically-induced substance antibody|Processed cyclic AMP-responsive element-binding protein 3-like protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CREB3L1 antibody, ASA-B0498, Western blotting
All lanes: Anti CREB3L1 (ASA-B0498) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
All lanes: Anti CREB3L1 (ASA-B0498) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
