More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cAMP responsive element binding protein 1 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CREB recombinant protein (Position: M1-T177). Human CREB shares 99% and 98% amino acid (aa) sequences identity with mouse and rat CREB, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CREB1 |
Protein | Cyclic AMP-responsive element-binding protein 1 |
Uniprot ID | P16220 |
Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the bZIP family. |
Aliases | Active transcription factor CREB antibody|cAMP response element binding protein 1 antibody|cAMP response element binding protein antibody|cAMP responsive element binding protein 1 antibody|cAMP-responsive element-binding protein 1 antibody|CREB 1 antibody|CREB antibody|CREB-1 antibody|CREB1 antibody|CREB1_HUMAN antibody|Cyclic AMP-responsive element-binding protein 1 antibody|MGC9284 antibody|OTTHUMP00000163864 antibody|OTTHUMP00000163865 antibody|OTTHUMP00000206660 antibody|OTTHUMP00000206662 antibody|OTTHUMP00000206667 antibody|Transactivator protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CREB antibody, ASA-B0497--1.jpg
All lanes: Anti CREB (ASA-B0497) at 0.5ug/ml
WB: Recombinant Human CREB Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti CREB (ASA-B0497) at 0.5ug/ml
WB: Recombinant Human CREB Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-CREB antibody, ASA-B0497--2.jpg
All lanes: Anti CREB (ASA-B0497) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 43KD
Observed bind size: 43KD
All lanes: Anti CREB (ASA-B0497) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 43KD
Observed bind size: 43KD
