More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| coxsackie virus and adenovirus receptor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Coxsackie Adenovirus Receptor(313-328aa YSKTQYNQVPSEDFER), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CXADR |
Protein | Coxsackievirus and adenovirus receptor(CAR/hCAR) |
Uniprot ID | P78310 |
Function | Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T- cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T- cells that in turn stimulate epithelial tissues repair. |
Tissue Specificity | Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. |
Sub-cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell junction, adherens junction. Basolateral cell membrane; Single-pass type I membrane protein. Note: In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. |
Sequence Similarities | |
Aliases | 46 kD coxsackievirus and adenovirus receptor CAR protein antibody|46 kD coxsackievirus and adenovirus receptor protein antibody|CAR antibody|CAR4/6 antibody|Coxsackie virus and adenovirus receptor antibody|Coxsackie virus B receptor antibody|Coxsackievirus and adenovirus receptor antibody|Coxsackievirus B adenovirus receptor antibody|Coxsackievirus B-adenovirus receptor antibody|CVB3 binding protein antibody|CVB3-binding protein antibody|Cxadr antibody|CXAR_HUMAN antibody|hCAR antibody|HCVADR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Coxsackie Adenovirus Receptor antibody, ASA-B0494, Western blotting
All lanes: Anti Coxsackie Adenovirus Receptor (ASA-B0494) at 0.5ug/ml
Lane 1: Rat Pancreas Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Heart Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: 293T Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 120KD
Observed bind size: 120KD
All lanes: Anti Coxsackie Adenovirus Receptor (ASA-B0494) at 0.5ug/ml
Lane 1: Rat Pancreas Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Heart Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: 293T Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 120KD
Observed bind size: 120KD
