ASA-B0474
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Collagen IV Antibody
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Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
collagen, type IV, alpha 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
A synthetic peptide corresponding to a sequence at the N-terminus of human Collagen IV(245-263aa QAQVQEKGDFATKGEKGQK), different from the related mouse and rat sequences by three amino acids. |
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Gene | COL4A1 |
Protein | Collagen alpha-1(IV) chain |
Uniprot ID | P02462 |
Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. |
Tissue Specificity | Highly expressed in placenta. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
Sequence Similarities | Belongs to the type IV collagen family. |
Aliases | Arresten antibody|Canstatin antibody|CO4A1_HUMAN antibody|COL4A1 antibody|COL4A1 NC1 domain antibody|Collagen Alpha 1(IV) Chain antibody|collagen alpha-1(IV) chain antibody|Collagen IV Alpha 1 Polypeptide antibody|Collagen Of Basement Membrane Alpha 1 Chain antibody|Collagen Type IV Alpha 1 antibody|DKFZp686I14213 antibody|FLJ22259 antibody |
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |