Collagen IV Antibody View larger

Collagen IV Antibody

ASA-B0474

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

collagen, type IV, alpha 1 Polyclonal IgG Rabbit Human WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Collagen IV(245-263aa QAQVQEKGDFATKGEKGQK), different from the related mouse and rat sequences by three amino acids.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

COL4A1

Protein

Collagen alpha-1(IV) chain

Uniprot ID

P02462

Function

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Tissue Specificity

Highly expressed in placenta.

Sub-cellular localization

Secreted, extracellular space, extracellular matrix, basement membrane.

Sequence Similarities

Belongs to the type IV collagen family.

Aliases

Arresten antibody|Canstatin antibody|CO4A1_HUMAN antibody|COL4A1 antibody|COL4A1 NC1 domain antibody|Collagen Alpha 1(IV) Chain antibody|collagen alpha-1(IV) chain antibody|Collagen IV Alpha 1 Polypeptide antibody|Collagen Of Basement Membrane Alpha 1 Chain antibody|Collagen Type IV Alpha 1 antibody|DKFZp686I14213 antibody|FLJ22259 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- COL4A1 antibody, ASA-B0474, Western blotting
All lanes: Anti COL4A1 (ASA-B0474) at 0.5ug/ml
Lane 1: MM231 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: SW620Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 160KD
Observed bind size: 160KD

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