More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| v-myc avian myelocytomatosis viral oncogene homolog | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human c-Myc recombinant protein (Position: E257-A439). Human c-Myc shares 91% amino acid (aa) sequence identity with both mouse and rat c-Myc. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MYC |
Protein | Myc proto-oncogene protein |
Uniprot ID | P01106 |
Function | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. |
Tissue Specificity | |
Sub-cellular localization | Nucleus, nucleoplasm. |
Sequence Similarities | Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases | AU016757 antibody|Avian myelocytomatosis viral oncogene homolog antibody|bHLHe39 antibody|c Myc antibody|c Myc Epitope Tag antibody|c Myc Tag antibody|Class E basic helix-loop-helix protein 39 antibody|MRTL antibody|Myc antibody|Myc Epitope Tag antibody|Myc protein antibody|Myc proto oncogene protein antibody|Myc proto-oncogene protein antibody|myc-related translation/localization regulatory factor antibody|MYC_HUMAN antibody|Myc2 antibody|MYCC antibody|Myelocytomatosis oncogene antibody|Niard antibody|Nird antibody|Oncogene Myc antibody|OTTHUMP00000158589 antibody|Proto-oncogene c-Myc antibody|Protooncogene homologous to myelocytomatosis virus antibody|RNCMYC antibody|Transcription factor p64 antibody|Transcriptional regulator Myc-A antibodyV-Myc avian myelocytomatosis viral oncogene homolog antibody|v-myc myelocytomatosis viral oncogene homolog (avian) antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-c-Myc antibody, ASA-B0253--1.jpg
All lanes: Anti C-MYC (ASA-B0253) at 0.5ug/ml
WB: Recombinant Human C-MYC Protein 0.5ng
Predicted bind size: 44KD
Observed bind size: 44KD
All lanes: Anti C-MYC (ASA-B0253) at 0.5ug/ml
WB: Recombinant Human C-MYC Protein 0.5ng
Predicted bind size: 44KD
Observed bind size: 44KD
Anti-c-Myc antibody, ASA-B0253--2.jpg
All lanes: Anti C-MYC (ASA-B0253) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Rat Thymus Tissue Lysate at 50ug
Lane 4: Rat Brain Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: HEPG2 Whole Cell Lysate at 40ug
Lane 9: K562 Whole Cell Lysate at 40ug
Pre
All lanes: Anti C-MYC (ASA-B0253) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Rat Thymus Tissue Lysate at 50ug
Lane 4: Rat Brain Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: HEPG2 Whole Cell Lysate at 40ug
Lane 9: K562 Whole Cell Lysate at 40ug
Pre
