More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| v-myb avian myeloblastosis viral oncogene homolog | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human c-Myb recombinant protein (Position: M1-E201). Human c-Myb shares 100% amino acid (aa) sequence identity with mouse c-Myb. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MYB |
Protein | Transcriptional activator Myb |
Uniprot ID | P10242 |
Function | Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 3 HTH myb-type DNA-binding domains. |
Aliases | C myb antibody|c myb protein (140 AA) antibody|c-myb protein (140 AA) antibody|c-myb_CDS antibody|c-myb10A_CDS antibody|c-myb13A_CDS antibody|c-myb14A_CDS antibody|c-myb8B_CDS antibody|Cmyb antibody|efg antibody|Myb antibody|MYB_HUMAN antibody|ONCOGENE AMV antibody|Oncogene MYB antibody|Proto oncogene c Myb antibody|Proto-oncogene c-Myb antibody|Transcriptional activator Myb antibody|v myb avian myeloblastosis viral oncogene homolog antibody|v-myb avian myeloblastosis viral oncogene homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- c-Myb antibody, ASA-B0250, Western blotting
All lanes: Anti c-Myb (ASA-B0250) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 74KD
Observed bind size: 74KD
All lanes: Anti c-Myb (ASA-B0250) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 74KD
Observed bind size: 74KD
