More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| claudin 5 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin 5(191-218aa RPDLSFPVKYSAPRRPTATGDYDKKNYV), different from the related mouse sequence by three amino acids and from the related rat sequence by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CLDN5 |
Protein | Claudin-5 |
Uniprot ID | O00501 |
Function | Plays a major role in tight junction-specific obliteration of the intercellular space. |
Tissue Specificity | |
Sub-cellular localization | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | Androgen withdrawal and apoptosis induced protein RVP1 like antibody|AWAL antibody|BEC 1 antibody|BEC1 antibody|Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody|Claudin-5 antibody|Claudin5 antibody|CLD5_HUMAN antibody|CLDN 5 antibody|CLDN5 antibody|CPETR L1 antibody|CPETRL 1 antibody|CPETRL1 antibody|TMDVCF antibody|TMVCF antibody|Transmembrane protein deleted in VCFS antibody|Transmembrane protein deleted in velocardiofacial syndrome antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Claudin 5 antibody, ASA-B0460, Western blotting
All lanes: Anti Claudin-5 (ASA-B0460) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti Claudin-5 (ASA-B0460) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 23KD
Observed bind size: 23KD
