CIAS1/NALP3  Antibody View larger

CIAS1/NALP3 Antibody

ASA-B0456

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

NLR family, pyrin domain containing 3 Polyclonal IgG Rabbit Human WB Immunogen affinity purified.

Immunogen

E.coli-derived human CIAS1 recombinant protein (Position: D21-K194). Human CIAS1 shares 77% amino acid (aa) sequence identity with mouse CIAS1.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

NLRP3

Protein

NACHT, LRR and PYD domains-containing protein 3

Uniprot ID

Q96P20

Function

May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.

Tissue Specificity

Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Belongs to the NLRP family.

Aliases

AGTAVPRL antibody|AII/AVP antibody|Angiotensin/vasopressin receptor AII/AVP like antibody|Angiotensin/vasopressin receptor AII/AVP-like antibody|C1orf7 antibody|Caterpiller protein 1.1 antibody|CIAS 1 antibody|CIAS1 antibody|CLR1.1 antibody|Cold autoinflammatory syndrome 1 antibody|Cold autoinflammatory syndrome 1 protein antibody|Cryopyrin antibody|Familial cold autoinflammatory syndrome antibody|FCAS antibody|FCU antibody|LRR and PYD domains-containing protein 3 antibody|Muckle-Wells syndrome antibody|MWS antibody|NACHT antibody|NACHT LRR and PYD containing protein 3 antibody|NALP 3 antibody|NALP3 antibody|NALP3_HUMAN antibody|NLRP3 antibody|PYPAF 1 antibody|PYPAF1 antibody|PYRIN containing APAF1 like protein 1 antibody|PYRIN-containing APAF1-like protein 1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CIAS1/NALP3 antibody, ASA-B0456--1.jpg
All lanes: Anti CIAS1 (ASA-B0456) at 0.5ug/ml
WB: Recombinant Human CIAS1 Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Anti-CIAS1/NALP3 antibody, ASA-B0456--2.jpg
All lanes: Anti CIAS1 (ASA-B0456) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 118KD
Observed bind size: 118KD

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