More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| baculoviral IAP repeat containing 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human cIAP2 recombinant protein (Position: M1-K191). Human cIAP2 shares 69.8% amino acid (aa) sequence identity with mouse cIAP2. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BIRC3 |
Protein | Baculoviral IAP repeat-containing protein 3 |
Uniprot ID | Q13489 |
Function | Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase- independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. |
Tissue Specificity | Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the IAP family. |
Aliases | AIP 1 antibody|AIP1 antibody|API 2 antibody|API2 antibody|API2 antibody| Apoptosis inhibitor 2 antibody|Baculoviral IAP repeat containing 3 antibody| Baculoviral IAP repeat containing protein 3 antibody|Baculoviral IAP repeat-containing protein 3 antibody|BIRC 3 antibody| BIRC3 antibody|BIRC3 antibody|BIRC3_HUMAN antibody|C IAP2 antibody|C-IAP2 antibody|CIAP 2 antibody|CIAP 2 antibody|CIAP2 antibody|HAIP 1 antibody|HAIP1 antibody| HAIP1 antibody|HIAP 1 antibody|HIAP-1 antibody|HIAP1 antibody|IAP homolog C antibody|IAP-1 antibody|Inhibitor of apoptosis protein 1 antibody|Inhibitor of apoptosis protein 1 antibody|MALT 2 antibody| MALT2 antibody|Mammalian IAP homolog C antibody|MIHC antibody|MIHC antibody|RING finger protein 49 antibody|RNF49 antibody|TNFR2 TRAF signaling complex protein 1 antibody| TNFR2 TRAF signalling complex protein antibody|TNFR2-TRAF-signaling complex protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- cIAP2 antibody, ASA-B0454, Western blotting
All lanes: Anti cIAP2 (ASA-B0454) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
All lanes: Anti cIAP2 (ASA-B0454) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
