More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| FBJ murine osteosarcoma viral oncogene homolog | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human c-Fos(170-187aa DQLEDEKSALQTEIANLL), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FOS |
Protein | Proto-oncogene c-Fos |
Uniprot ID | P01100 |
Function | Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. Note: In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30. |
Sequence Similarities | Belongs to the bZIP family. Fos subfamily. |
Aliases | Activator protein 1 antibody|AP 1 antibody|Cellular oncogene c fos antibody|Cellular oncogene fos antibody|FBJ murine osteosarcoma viral(v fos) oncogene homolog(oncogene FOS) antibody|FBJ murine osteosarcoma viral v fos oncogene homolog antibody|FBJ Osteosarcoma Virus antibody|FOS antibody|FOS protein antibody|FOS_HUMAN antibody|G0 G1 switch regulatory protein 7 antibody|G0/G1 switch regulatory protein 7 antibody|G0S7 antibody|Oncogene FOS antibody|p55 antibody|proto oncogene c Fos antibody|Proto oncogene protein c fos antibody|Proto-oncogene c-Fos antibody|v fos FBJ murine osteosarcoma viral oncogene homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-c-Fos antibody, ASA-B0248, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
