More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cholesteryl ester transfer protein, plasma | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CETP(368-382aa PRPDQQHSVAYTFEE). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CETP |
Protein | Cholesteryl ester transfer protein |
Uniprot ID | P11597 |
Function | Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL (PubMed:3600759, PubMed:24293641). Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination (PubMed:17237796). |
Tissue Specificity | Expressed by the liver and secreted in plasma. |
Sub-cellular localization | Secreted, extracellular space. |
Sequence Similarities | Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. |
Aliases | BPIFF antibody|CETP antibody|CETP_HUMAN antibody|Cholesteryl ester transfer antibody|Cholesteryl ester transfer protein antibody|Cholesteryl ester transfer protein plasma antibody|Cholesteryl ester transfer protein precursor antibody|HDLCQ10 antibody|Lipid transfer protein I antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CETP antibody, ASA-B0448, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: RAJI Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: RAJI Cell Lysate
Lane 6: MCF-7 Cell Lysate
