More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| centromere protein A | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CENPA recombinant protein (Position: M1-G140). Human CENPA shares 71.6% amino acid (aa) sequence identity with mouse CENPA. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CENPA |
Protein | Histone H3-like centromeric protein A |
Uniprot ID | P49450 |
Function | Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro). |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Chromosome, centromere, kinetochore. Note: Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height. |
Sequence Similarities | |
Aliases | CENP A antibody|CENP-A antibody|cenpa antibody|CENPA_HUMAN antibody| Centromere autoantigen A antibody|Centromere protein A 17kDa antibody| Centromere protein A antibody|Histone H3 like centromeric protein A antibody| Histone H3-like centromeric protein A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CENPA antibody, ASA-B0447, Western blotting
All lanes: Anti CENPA (ASA-B0447) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Pancreas Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 16KD
All lanes: Anti CENPA (ASA-B0447) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Pancreas Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 16KD
