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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cyclin-dependent kinase 6 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk6(291-310aa AYSALSHPYFQDLERCKENL), different from the related rat and mouse sequences by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDK6 |
Protein | Cyclin-dependent kinase 6 |
Uniprot ID | Q00534 |
Function | Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663). |
Tissue Specificity | Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells. |
Sub-cellular localization | Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
Sequence Similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. |
Aliases | CDK 6 antibody|CDK-6 antibody|CDK6 antibody|CDK6_HUMAN antibody|Cell division protein kinase 6 antibody|Crk 2 antibody|Crk2 antibody|Cyclin dependent kinase 6 antibody|Cyclin-dependent kinase 6 antibody|MGC59692 antibody|p40 antibody|PLSTIRE antibody|Serine/threonine protein kinase PLSTIRE antibody|Serine/threonine-protein kinase PLSTIRE antibody|STQTL11 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cdk6 antibody, ASA-B0437, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: COLO320 Cell Lysate
