More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cell division cycle 5-like | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CDC5L(782-802aa KELQHRYADLLLEKETLKSKF), different from the related rat and mouse sequences by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDC5L |
Protein | Cell division cycle 5-like protein |
Uniprot ID | Q99459 |
Function | DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). |
Tissue Specificity | Ubiquitously expressed in both fetal and adult tissues. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the CEF1 family. |
Aliases | CDC5 antibody|CDC5 cell division cycle 5-like(S. pombe) antibody|Cdc5 related protein antibody|CDC5-LIKE antibody|CDC5-like protein antibody|CEF1 antibody|Cell division cycle 5 S. pombe homolog of antibody|Cell division cycle 5-like protein antibody|dJ319D22.1 antibody|KIAA0432 antibody|PCDC5RP antibody|Pombe Cdc5 related protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CDC5L antibody, ASA-B0423, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: RAJI Cell Lysate
Lane 3: A549 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: RAJI Cell Lysate
Lane 3: A549 Cell Lysate
