More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cell division cycle 25 homolog C(S. pombe) | Monoclonal | IgG | Mouse | Human | WB | Ascites |
Immunogen |
| Recombinant human Cdc25c. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDC25C |
Protein | M-phase inducer phosphatase 3 |
Uniprot ID | Human(P30307), Mouse(P48967), Rat(D4ADT2) |
Function | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the MPI phosphatase family. |
Aliases | CDC25|PPP1R60|MITOSIS INDUCER CDC25|cell division cycle 25C|protein phosphatase 1, regulatory subunit 60|Dual specificity phosphatase Cdc25C |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 1-2μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
