More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| selectin L | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse CD62L(125-141aa KEDCVEIYIKRERDSGK), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SELL |
Protein | L-selectin |
Uniprot ID | P18337 |
Function | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (By similarity). |
Tissue Specificity | Predominantly expressed in lymphoid tissue. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the selectin/LECAM family. |
Aliases | A.11 antibody|AI528707 antibody|CD62 antigen ligand antibody|CD62 antigen-like family member L antibody|CD62L antibody|gp90 MEL antibody|gp90-MEL antibody|hLHRc antibody|IgA nephropathy, susceptibility to, included antibody|L Selectin antibody|L-selectin antibody|LAM-1 antibody|LAM1 antibody|LECAM1 antibody|LEU8 antibody|Leukocyte adhesion molecule 1 antibody|Leukocyte surface antigen Leu-8 antibody|Leukocyte surface antigen Leu8 antibody|Leukocyte-endothelial cell adhesion molecule 1 antibody|LNHR antibody|LSEL antibody|Ly-22 antibody|Ly-m22 antibody|Lyam-1 antibody|LYAM1 antibody|LYAM1_HUMAN antibody|Lymph node homing receptor antibody|Lymphocyte adhesion molecule 1 antibody|Lymphocyte antigen 22 antibody|Lymphocyte surface MEL-14 antigen antibody|MEL-14 antibody|Pln homing receptor antibody|PLNHR antibody|Selectin L antibody|Selectin, lymphocyte antibody|SELL antibody|TQ1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD62L antibody, ASA-B0393, Western blotting
Lane 1: Recombinant Mouse L-Selectin Protein 10ng
Lane 2: Recombinant Mouse L-Selectin Protein 5ng
Lane 3: Recombinant Mouse L-Selectin Protein 2.5ng
Lane 1: Recombinant Mouse L-Selectin Protein 10ng
Lane 2: Recombinant Mouse L-Selectin Protein 5ng
Lane 3: Recombinant Mouse L-Selectin Protein 2.5ng
