More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD40 molecule, TNF receptor superfamily member 5 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CD40(20-37aa, PEPPTACREKQYLINSQC). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD40 |
Protein | Tumor necrosis factor receptor superfamily member 5 |
Uniprot ID | P25942 |
Function | Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. |
Tissue Specificity | B-cells and in primary carcinomas. |
Sub-cellular localization | Isoform I: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 4 TNFR-Cys repeats. |
Aliases | AI326936 antibody|B cell associated molecule CD40 antibody|B cell surface antigen CD40 antibody|B cell-associated molecule antibody|B-cell surface antigen CD40 antibody|Bp50 antibody|CD 40 antibody|CD40 antibody|CD40 antigen(TNF receptor superfamily member 5) antibody|CD40 antigen antibody|CD40 molecule antibody|CD40 molecule, TNF receptor superfamily member 5 antibody|CD40 protein antibody|CD40 type II isoform antibody|CD40L receptor antibody|CDw40 antibody|GP39 antibody|HIGM1 antibody|IGM antibody|IMD3 antibody|MGC9013 antibody|Nerve growth factor receptor related B lymphocyte activation molecule antibody|OTTHUMP00000031699 antibody|OTTHUMP00000031700 antibody|p50 antibody|T-BAM antibody|TBAM antibody|TNF receptor superfamily member 5 antibody|TNFRSF5 antibody|TNR5_HUMAN antibody|TRAP antibody|Tumor necrosis factor receptor superfamily , member 5 antibody|Tumor necrosis factor receptor superfamily member 5 antibody|Tumor necrosis factor receptor superfamily member 5 precursor antibody|Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD40 antibody, ASA-B0380, Western blotting
Lane 1: Recombinant Human CD40 Protein 10ng
Lane 2: Recombinant Human CD40 Protein 5ng
Lane 3: Recombinant Human CD40 Protein 2.5ng
Lane 1: Recombinant Human CD40 Protein 10ng
Lane 2: Recombinant Human CD40 Protein 5ng
Lane 3: Recombinant Human CD40 Protein 2.5ng
