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CD31 Antibody

ASA-B0374

$330.00

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Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
platelet/endothelial cell adhesion molecule 1 Polyclonal IgG Rabbit Mouse WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse CD31(547-566aa HNKQASKKQEGQYYCTASNR).

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 PECAM1

Protein

 Platelet endothelial cell adhesion molecule

Uniprot ID

 Q08481

Function

 Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation (By similarity). Induces susceptibility to atherosclerosis.

Tissue Specificity

Isoform 1 and isoform 3 are expressed in lung and platelets.

Sub-cellular localization

 Cell membrane ; Single-pass type I membrane protein . Cell membrane ; Lipid-anchor . Cell junction. Note: Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

Sequence Similarities

 Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Aliases

 Adhesion molecule antibody|CD31 antibody|CD31 antigen antibody|CD31 EndoCAM antibody|Endocam antibody|FLJ34100 antibody|FLJ58394 antibody|GPIIA antibody|GPIIA' antibody|PECA1 antibody|PECA1_HUMAN antibody|Pecam 1 antibody|PECAM 1 CD31 EndoCAM antibody|PECAM antibody|PECAM-1 antibody|Pecam1 antibody|Platelet endothelial cell adhesion molecule antibody|Platelet/endothelial cell adhesion molecule 1 antibody|Platelet/endothelial cell adhesion molecule antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml MouseAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD31 antibody, ASA-B0374, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Mouse PECAM1, 46.2KD(162aa tag+ P474-T727
Lane 1: Recombinant Mouse PECAM1 Protein 10ng
Lane 2: Recombinant Mouse PECAM1 Protein 5ng
Lane 3: Recombinant Mouse PECAM1 Protein 2.5ng
Lane 4: Recombinant Mouse PECAM1 Protein 1.25ng

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