More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD274 molecule | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CD274(100-114aa QITDVKLQDAGVYRC), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD274 |
Protein | Programmed cell death 1 ligand 1(PD-L1/PDCD1 ligand 1/Programmed death ligand 1) |
Uniprot ID | Q9NZQ7 |
Function | Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. |
Tissue Specificity | Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. |
Sub-cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the immunoglobulin superfamily. BTN/MOG family. |
Aliases | B7 H antibody|B7 H1 antibody|B7 homolog 1 antibody|B7-H1 antibody|B7H antibody|B7H1 antibody|CD 274 antibody|Cd274 antibody|CD274 antigen antibody|CD274 molecule antibody|MGC142294 antibody|MGC142296 antibody|OTTHUMP00000021029 antibody|PD L1 antibody|PD-L1 antibody|PD1L1_HUMAN antibody|PDCD1 ligand 1 antibody|PDCD1L1 antibody|PDCD1LG1 antibody|PDL 1 antibody|PDL1 antibody|Programmed cell death 1 ligand 1 antibody|Programmed death ligand 1 antibody|RGD1566211 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD274 antibody, ASA-B0369, Western blotting
All lanes: Anti CD274 (ASA-B0369) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 70KD
All lanes: Anti CD274 (ASA-B0369) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 70KD
