More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD2 molecule | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse CD2(224-242aa FIFCICKRRKRNRRRKDEE), different from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD2 |
Protein | T-cell surface antigen CD2 |
Uniprot ID | P08920 |
Function | CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function. |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
Aliases | CD 2 antibody|CD2 antibody|CD2 antigen(p50), sheep red blood cell receptor antibody|CD2 antigen antibody|CD2 molecule antibody|CD2_HUMAN antibody|Erythrocyte receptor antibody|FLJ46032 antibody|LFA-2 antibody|LFA-3 receptor antibody|LFA2 antibody|LFA3 receptor antibody|Ly-37 antibody|Lymphocyte function antigen 2 antibody|lymphocyte-function antigen-2 antibody|OTTHUMP00000024366 antibody|Rosette receptor antibody|SRBC antibody|T cell surface antigen CD2 antibody|T-cell surface antigen CD2 antibody|T-cell surface antigen T11/Leu-5 antibody|T-lymphocyte surface CD2 antigen antibody|T11 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD2 antibody, ASA-B0363, Western blotting
Lane 1: Rat Spleen Cell Lysate
Lane 2: NIH/3T3 Cell Lysate
Lane 1: Rat Spleen Cell Lysate
Lane 2: NIH/3T3 Cell Lysate
