More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| basigin (Ok blood group) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CD147 recombinant protein (Position: E53-S385). Human CD147 shares 64% and 65% amino acid (aa) sequence identity with mouse and rat CD147, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | 575 |
Protein | Basigin |
Uniprot ID | P35613 |
Function | Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. |
Tissue Specificity | Present only in vascular endothelium in non- neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
Aliases | 5A11 antigen antibody|5F7 antibody|BASI_HUMAN antibody|Basigin (Ok blood group) antibody|Basigin antibody|Blood brain barrier HT7 antigen antibody|BSG antibody|CD 147 antibody|CD147 antibody|CD147 antigen antibody|Collagenase stimulatory factor antibody|Emmprin antibody|Extracellular matrix metalloproteinase inducer antibody|Leukocyte activation antigen M6 antibody|M 6 antibody|M6 antibody|M6 leukocyte activation antigen antibody|Neurothelin antibody|OK antibody|OK blood group antibody|OK blood group antigen antibody|TCSF antibody|Tumor cell derived collagenase stimulatory factor antibody|Tumor cell-derived collagenase stimulatory factor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CD147 antibody, ASA-B0354, Western blotting
All lanes: Anti CD147 (ASA-B0354) at 0.5ug/ml
WB: Recombinant Human CD147 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti CD147 (ASA-B0354) at 0.5ug/ml
WB: Recombinant Human CD147 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- CD147 antibody, ASA-B0354, Western blotting
All lanes: Anti CD147 (ASA-B0354) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: U20S Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
All lanes: Anti CD147 (ASA-B0354) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: U20S Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
