More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| integrin, alpha M(complement component 3 receptor 3 subunit) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CD11b(177-192aa MEQLKKSKTLFSLMQY), different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ITGAM |
Protein | Integrin alpha-M |
Uniprot ID | P11215 |
Function | Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. |
Tissue Specificity | Predominantly expressed in monocytes and granulocytes. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the integrin alpha chain family. |
Aliases | Antigen CD11b p170 antibody|CD11 antigen like family member B antibody|CD11 antigen-like family member B antibody|CD11b antibody|CD11b antigen antibody|CD11b/CD18 antibody|CD49d antibody|Cell surface glycoprotein MAC 1 alpha subunit antibody|Cell surface glycoprotein MAC-1 subunit alpha antibody|Cell Surface Glycoprotein MAC1 Alpha Subunit antibody|Complement component 3 receptor 3 subunit antibody|Complement Component Receptor 3 Alpha antibody|Complement receptor type 3, alpha subunit antibody|CR 3 alpha chain antibody|CR-3 alpha chain antibody|CR3 Alpha Chain antibody|CR3 antibody|CR3A antibody|F730045J24Rik antibody|Integrin Alpha M antibody|Integrin alpha M chain antibody|Integrin alpha M complement component 3 receptor 3 subunit antibody|Integrin alpha-M antibody|Integrin beta 2 alpha subunit antibody|ITAM_HUMAN antibody|ITGAM antibody|Leukocyte Adhesion Receptor MO1 antibody|Ly-40 antibody|MAC 1 antibody|Mac-1a antibody|MAC1 antibody|Mac1, alpha subunit antibody|MAC1A antibody|Macrophage antigen alpha polypeptide antibody|MGC117044 antibody|Mo1, alpha subunit antibody|MO1A antibody|Neutrophil adherence receptor alpha M subunit antibody|Neutrophil Adherence Receptor antibody|SLEB6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD11b antibody, ASA-B0344, All Western blotting
All lanes: Anti-ITGAM(ASA-B0344) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 170KD
All lanes: Anti-ITGAM(ASA-B0344) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 170KD
