More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cysteine-rich, angiogenic inducer, 61 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CCN1 recombinant protein (Position: K54-D381). Human CCN1 shares 91.8% and 92.1% amino acid (aa) sequence identity with mouse and rat CCN1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYR61 |
Protein | Protein CYR61 |
Uniprot ID | O00622 |
Function | Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the CCN family. |
Aliases | 3CH61 antibody|CCN 1 antibody|CCN family member 1 antibody|CCN1 antibody|CCN1 antibody|CYR 61 antibody|Cyr61 antibody|CYR61 protein antibody|CYR61 protein precursor antibody|CYR61 protein precursor antibody| CYR61_HUMAN antibody|Cysteine rich angiogenic inducer 61 antibody| Cysteine rich angiogenic inducer 61 antibody|Cysteine rich angiogenic inducer 61 antibody|Cysteine rich heparin binding protein 61 antibody|Cysteine-rich angiogenic inducer 61 antibody|GIG 1 antibody|GIG1 antibody|GIG1 protein antibody|GIG1 protein antibody|IBP-10 antibody|IGF-binding protein 10 antibody|Igfbp 10 antibody|IGFBP-10 antibody|IGFBP10 antibody|IGFBP10 antibody|Insulin like growth factor binding protein 10 antibody|Insulin like growth factor binding protein 10 antibody|Insulin like growth factor binding protein 10 antibody|Insulin-like growth factor-binding protein 10 antibody| Protein CYR61 antibody|Protein GIG1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CCN1 antibody, ASA-B0331, Western blotting
All lanes: Anti CCN1 (ASA-B0331) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: A431 Whole Cell Lysate at 40ug
Lane 4: SGC Whole Cell Lysate at 40ug
Lane 5: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 42KDg
Observed bind size: 42KD
All lanes: Anti CCN1 (ASA-B0331) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: A431 Whole Cell Lysate at 40ug
Lane 4: SGC Whole Cell Lysate at 40ug
Lane 5: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 42KDg
Observed bind size: 42KD
