CCL3  Antibody View larger

CCL3 Antibody

ASA-B0327

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

chemokine (C-C motif) ligand 3 Polyclonal IgG Rabbit Rat ELISA, WB Immunogen affinity purified.

Immunogen

E.coli-derived rat CCL3 recombinant protein (Position: A24-A92). Rat CCL3 shares 74% and 91% amino acid (aa) sequences identity with human and mouse CCL3, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

CCL3

Protein

C-C motif chemokine 3

Uniprot ID

P50229

Function

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

Tissue Specificity

Sub-cellular localization

Secreted.

Sequence Similarities

Aliases

C C motif chemokine 3 antibody|CCL 3 antibody|CCL3 antibody|CCL3_HUMAN antibody|Chemokine C|C motif ligand 3 antibody|Chemokine CC Motif Ligand 3 antibody|G0/G1 switch regulatory protein 19 1 antibody|G0/G1 switch regulatory protein 19-1 antibody|G0S19 1 antibody|G0S19 1 protein antibody|Heparin binding chemotaxis protein antibody|L2G25B antibody|LD78 alpha antibody|LD78-alpha(4-69) antibody|LD78ALPHA antibody|Macrophage inflammatory protein 1 alpha antibody|Macrophage inflammatory protein 1-alpha antibody|MIP 1 alpha antibody|MIP 1A antibody|MIP-1-alpha antibody|MIP-1-alpha(4-69) antibody|MIP1A antibody|PAT 464.1 antibody|SCYA 3 antibody|SCYA3 antibody|SIS alpha antibody|SIS beta antibody|SIS-beta antibody|Small inducible cytokine A3 antibody|Small-inducible cytokine A3 antibody|Tonsillar lymphocyte LD78 alpha protein antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml RatAssaySolutio's ECL kit
ELISA 0.1-0.5μg/ml RatSandwich ELISA format

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CCL3 antibody, ASA-B0327--1.jpg
All lanes: Anti-CCL3(ASA-B0327) at 0.5ug/ml
WB: Recombinant Rat CCL3 Protein 0.5ng
Predicted bind size: 33KD
Observed bind size: 33KD

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